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nikon a1 confocal microscope  (Nikon)


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    Nikon nikon a1 confocal microscope
    Nikon A1 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+elements/NIS-Elements/pmc12966746-90-7-7
    Average 99 stars, based on 39798 article reviews
    nikon a1 confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Software:

    Article Title: C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin
    Article Snippet: Software, algorithm , FlowJo , https://www.flowjo.com/ , , Flow cytometry analysis. .. Software, algorithm , Nikon Elements , https://www.microscope.healthcare.nikon.com/products/software/nis-elements , , Image acquisition. .. Software, algorithm , GraphPad Prism , https://www.graphpad.com , , Statistical tests.

    Article Title: Identification of presenilin mutations that have sufficient gamma-secretase proteolytic activity to mediate Notch signaling but disrupt organelle and neuronal health.
    Article Snippet: .. Animals were mounted on 3 % agarose pads with 0.1 % tetramisole and imaged using Nikon Elements (Version AR 5.21.03) software on a Nikon A1R confocal microscope equipped with a 60× oil objective lens. ..

    Article Title: Identification of presenilin mutations that have sufficient gamma-secretase proteolytic activity to mediate Notch signaling but disrupt organelle and neuronal health.
    Article Snippet: Animals were mounted on 3 % agarose pads with 0.1 % tetramisole and imaged on a Nikon Eclipse Ti2 microscope using 60× oil objective lens. .. The images were captured with a Teledyne Prime BSI sCMOS camera using Nikon Elements (Version AR 5.42.03) software. .. For all samples, GCaMP6f fluorescence was excited by using 488 nm light at 1 % power on a Lumencor Spectra Light Engine and emitted light was collected via a 525/25 m band pass filter (Chroma) with an exposure time of 80 ms. For all samples, wrmScarlet fluorescent was excited by using 561 nm at 3.5 % power on a Lumencor Spectra Light Engine and emitted light was collected via a 635/60 nm band pass filter (Chroma) with an exposure time of 200 ms. Post hoc fluorescence intensities were determined blind to genotype using Fiji (Version 1.54f), with GCaMP6f fluorescence intensity/area normalized to wrmScarlet fluorescence intensity/area.

    Article Title: Identification of presenilin mutations that have sufficient gamma-secretase proteolytic activity to mediate Notch signaling but disrupt organelle and neuronal health.
    Article Snippet: GFP fluorescence was excited using 488 nm light source at 1 % power on a Lumencor Spectra Light Engine and emitted light collected via a 525/25 nm band pass filter (Chroma). .. The GFP epifluorescence images were captured with a Teledyne Prime BSI sCMOS camera with an exposure time set to 100 ms using Nikon Elements (Version AR 5.42.03) software. ..

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD.
    Article Snippet: Brian J. Joseph , Kelly A. Marshall , Peter Harley , Jacob R. Mann, Francesco Alessandrini, Carlos G. Vanoye, Wanhao Chi , Mercedes Prudencio , Dina Simkin , Tzu-Ting Kao , Reshma R. Desai, Matthew J. Keuss , Simone Barattucci , Matteo Zanovello, Puja R. Mehta , Jean-Marc DeKeyser, Francesco Limone, Jonathan Lee , Anna-Leigh Brown, Marcel F. Leyton-Jaimes , Leslie A. Nash, Irune Guerra San Juan, Eleonora Aronica , Brian J. Wainger , Mala Shah, Anand Goswami, Neil A. Shneider, Dennis W. Dickson , Juan Burrone, Chaolin Zhang , Hynek Wichterle , Leonard Petrucelli, Jonathan K. Watts , Alfred L. George Jr, Pietro Fratta , Kevin Eggan & Evangelos Kiskinis

    Imaging:

    Article Title: Basal autophagy during meiotic prophase I is required for accurate chromosome segregation in Drosophila oocytes and declines during oocyte aging
    Article Snippet: .. All imaging was performed using an Andor spinning disc confocal (50 μm pinhole) running Nikon Elements (5.11.02 Build 1369) to operate a Nikon Eclipse Ti inverted microscope, with an ASI MS 2000 motorized piezo stage, Zyla 4.2-megapixel sCMOS camera and four lasers (405, 561, 531 and 637 nm). ..

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD.
    Article Snippet: Brian J. Joseph , Kelly A. Marshall , Peter Harley , Jacob R. Mann, Francesco Alessandrini, Carlos G. Vanoye, Wanhao Chi , Mercedes Prudencio , Dina Simkin , Tzu-Ting Kao , Reshma R. Desai, Matthew J. Keuss , Simone Barattucci , Matteo Zanovello, Puja R. Mehta , Jean-Marc DeKeyser, Francesco Limone, Jonathan Lee , Anna-Leigh Brown, Marcel F. Leyton-Jaimes , Leslie A. Nash, Irune Guerra San Juan, Eleonora Aronica , Brian J. Wainger , Mala Shah, Anand Goswami, Neil A. Shneider, Dennis W. Dickson , Juan Burrone, Chaolin Zhang , Hynek Wichterle , Leonard Petrucelli, Jonathan K. Watts , Alfred L. George Jr, Pietro Fratta , Kevin Eggan & Evangelos Kiskinis

    Inverted Microscopy:

    Article Title: Basal autophagy during meiotic prophase I is required for accurate chromosome segregation in Drosophila oocytes and declines during oocyte aging
    Article Snippet: .. All imaging was performed using an Andor spinning disc confocal (50 μm pinhole) running Nikon Elements (5.11.02 Build 1369) to operate a Nikon Eclipse Ti inverted microscope, with an ASI MS 2000 motorized piezo stage, Zyla 4.2-megapixel sCMOS camera and four lasers (405, 561, 531 and 637 nm). ..

    Microscopy:

    Article Title: Identification of presenilin mutations that have sufficient gamma-secretase proteolytic activity to mediate Notch signaling but disrupt organelle and neuronal health.
    Article Snippet: .. Animals were mounted on 3 % agarose pads with 0.1 % tetramisole and imaged using Nikon Elements (Version AR 5.21.03) software on a Nikon A1R confocal microscope equipped with a 60× oil objective lens. ..



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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Obtained images were processed and analyzed using Nikon NIS Elements software.

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software

    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Three-dimensional structures were reconstructed using NIS Elements imaging software for Nikon microscopy (Nikon, Japan).

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software